Gram stain is still used in medical ...

Gram staining method was developed and implemented by Hans Christian Gram, published in 1884. The method is based on the chemical composition of the cell wall of bacteria. Due to differences in the chemical structure of the cell wall is not less than 4 grams of colored organisms are defined by: Gram-positive, Gram-negative, Gram variable and Gram-negative groups unknown. This technique is used as a tool to distinguish between two major groups of bacteria: Gram-positive and Gram-negative in the microbiology laboratory and medical diagnosis of bacterial infections. With current technology, environmental microbiology using other methods, e. , the PCR tests

DNA genetic markers to identify the various bacteria. Gram is still used in medical microbiology, as a quick way to get some idea of ​​the bacteria involved in the infectious process. Its advantage is the speed of the result to 24 or more hours in anticipation of bacterial cultures. Thus, patients with suspected bacterial infection have body fluid or biopsy material stained with Gram's method, as well as microbiological cultures. bacteria include proetobacteria, cyanobacteria, spirochetes, green sulfur and green

no sulfur bacteria. The group also contains relevant medical

Gram-negative cocci, bacilli, and many bacteria associated with nosocomial infections. These organisms stain pink and are a source of endotoxin. Bacillus, Staphylococcus, Streptococcus, Enterococcus,

diplococcus pneumonia, Clostridium. These organisms stain purple. Partly a result of bacteria in the mixed form of color: pink and purple, because their cell walls, are particularly susceptible to breakage, resulting in a negative and gram positive and gram-negative cells. The spot method uses the structure of the bacterial cell wall. Gram-positive bacteria

thick cell wall peptiglycan strattera 10mg, which stains purple. Gram-negative bacteria

aa thin layer of the cell wall (stained pink), and they also have an outer layer containing lipids. Staining method involves four major steps: (1) smear slide culture stained with crystal violet in the heart (Bunsen burner). 2) the capture agent) Gram iodine) is used next. (3) The rapid de-

color is seen by placing the slide (s) in alcohol or acetone. 4) Counter staining of safranin done, and sometimes basic fuchsin used. damage to structures. Personally, PCR-DNA tests

probably more reliable for identification of bacterial species. However, the latter test is expensive, and the Gram method provides no information about which group the bacteria belong to the most environmentally. .